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Sudden Oak Death The "Bottle O' Bait" (BOB) method for detecting P. ramorum in water
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Sammamish River in Bothell. This is a fairly slow moving river that gets water inputs from residential, agricultural, and commercially developed areas.
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Fishing for bait bag at site 13. A pole is useful when bait bags get carried away by the current.
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Collecting water at site 13A ditch
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Woodin Creek – site 21 (it was getting dark)
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Preparing water samples for BOB and filtering
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Filtration apparatus in lab
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Collecting water at site 7. Water samples are collected in 100 ml aliquots and mixed, rather than collecting the entire 1L at once. This method works better in cases where inoculum density is low.
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Ditch at site 13A – industrial site
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Little Bear Creek – site 12. This creek was swollen from recent rainfall.
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Collecting leaves from bait bag at Gig Harbor site
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BOB setup. A whole Rhododendron leaf (not shown) is also added to the bottle containing 1L stream water and 20 small leaf baits. These baits are incubated in stream water and then plated onto Phytophthora selective media.
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Culturing filter on selective media