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Sudden Oak Death Plant Pathology

“The shoe experiment” – testing various disinfectants for sanitizing the bottoms of shoes


”The shoe experiment” – testing various disinfectants for sanitizing the bottoms of shoes

Students from Pierce College, Puyallup, investigated whether some commonly used disinfectants are able to kill P. ramorum inoculum transported on shoes. It is a common practice to spray shoe soles with a disinfectant when leaving a P. ramorum positive nursery or lab where P. ramorum is studied, but there has been little research showing which disinfectants are effective in reducing inoculum.

In this study, three types of shoe material and six treatments were tested. Three of the treatments were effective in destroying P. ramorum inoculum, however this was decreased in the presence of soil. Therefore, removing soil from shoes before spraying with disinfectants is recommended.

 

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shoe results

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Understanding Phytophthora and Pythium species found in streams


Understanding Phytophthora & Pythium species found in streams

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General information

Five Questions About Oomycetes Small Things Considered, the microbe blog, November 16, 2009.

Introduction to the Oomycota University of California Museum of Palentology

Oomycetes in the news

For some, pythium blight is a job-killer. The Olympian May 17, 2011

Journal articles on Phytophthora and Pythium taxonomy

Beakes GW, Glockling SL, Sekimoto S. 2011. The evolutionary phylogeny of the oomycete “fungi”. Protoplasma DOI 10.1007/s00709-011-0269-2

BRASIER,C.M., COOKE,D. E. L., DUNCAN,J. M., and HANSEN,E. M.
2003. Multiple new phenotypic taxa from trees and riparian ecosystems in Phytophthora gonapodyides – P. megasperma ITS Clade 6, which tend to be high-temperature tolerant and either inbreeding or sterile
http://dx.doi.org/10.1017/S095375620300738X

Burgess, T.I., Stukely, M.J.C., Jung, T., White, D., Hüberli, D. and Hardy, G.E.St.J. (2010) Molecular characterisation of a Phytophthora hybrid swarm in native ecosystems and waterways in Western Australia. In: 5th IUFRO Phytophthora Diseases in Forests and Natural Ecosystems, 7 – 12 March, Auckland and Rotorua, New Zealand.

Cook, K.L., Hudspeth, D.S.S., and Hudspeth, M.E.S. 2001. A cox2 phylogeny of representative marine peronosporomycetes (Oomycetes). Nova Hedwigia 122: 231-243.

Ecology of aquatic oomycetes

Kageyama. K. Assessment of river environment using Pythium species. A presentation on a study relating Pythium spp. to land use.

Nakagiri, A. (2000). Ecology and diversity of Halophytophthora species. In: Aquatic Mycology across the Millennium (eds K.D. Hyde, W.H. Ho and S.B. Pointing). Fungal Diversity 5: 153164.

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Puget Creek-1005


Puget

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Creek

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Site # 1005

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Baiting at Puget Creek March 31, 2010 by Environmental Microbiology class (TESC 378) from UW-Tacoma.

Results

pugetcrkresults

Links

Puget Creek Restoration Society

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Intensively sampled sites


Intensively sampled sites

Three sites were chosen to test whether there is a difference between Rhododendron species used as leaf baits. The sites were First Creek (1004), Puget Creek (1005), and Clarks Creek headwaters (1007) Rhododendron macrophyllum and R. ‘Nova Zembla’ were compared. More samples per site were collected to better evaluate Phytophthora species diversity.

Sites were monitored for one baiting period using 40 bait leaves, 20 from each Rhododendron species. Two samples per leaf were isolated and identified, for a total of 80 per site.

There were no differences in Phytophthora species isolated from the two types of bait leaves, however there were significant differences among sites.

Wild Card Plants-Long Term Sites


Extra Plants

On the long-term monitoring sites, volunteers chose an extra plant species to be used as bait. Two samples from each plant were cultured and identified:

Baiting period 1001 Clarks Creek Downstream 1002 Anderson Creek 1003 Evans Creek 1006 Dogfish Creek
1
 Camellia
P. gonapodyides (1), unknown (1)		 Sword fern (Polystichum munitum)
P. gonapodyides (1), unknown (1)		 Himalayan blackberry (Rubus armeniacus)
P. gonapodyides (2)		 English laurel (Prunus laurocerasus)
no growth
2
 Gold dust plant (Aucuba japonica ‘Variegata)
Pythium litorale or Py. sterilum (2)		 Sword fern (Polystichum munitum)
P. gonapodyides (1), unknown (1)		 Japanese knotweed (Polygonum cuspidatum)
P. gonapodyides (2)		 Viburnum davidii
unknown (2)
3
 Strawberry tree (Arbutus unedo)
Pythium sp. or Py. undulatum (1), unknown (1)		 Sword fern (Polystichum munitum)
Pythium sp. or Py. undulatum (1), unknown (1)		 English laurel (Prunus laurocerasus)
fungus* (Ophiocordyceps crassispora, Cylindrocarpon obtusisporum, Nectria) (1), unknown (1)		 English ivy (Hedera helix )P. gonapodyides (2), unknown (1)
4
 Dwarf cherry laurel P. gonapodyides (2), unknown (2) Dandelion (Taraxacum officinale)unknown (2) Pacific madrone (Arbutus menziesii)unknown (2) Rhododendron spp. P. gonapodyides (1), unknown (1)
5
 unknown (2) Horsetail (Equisetum spp.)unknown (2) Evergreen huckleberry (Vaccinium ovatum)P. gonapodyides (1), unknown (2) English laurel (Prunus laurocerasus)
6
 Red tip Photinia P. gonapodyides (2) Red alder (Alnus rubra)P. gonapodyides (1), unknown (2) Kousa dogwood (Cornus kousa)no growth English ivy (Hedera helix )unknown (2)

*probably endophytic in host leaf

Dogfish Creek


Dogfish

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Creek

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Site # 1006

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 First bait deployment at Dogfish Creek by Master Gardener volunteers, March 9, 2010. Poulsbo, WA

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Baiting period Bait leaf spp. Phytophthora spp. and others identified
1
 Rhododendron P. gonapodyides (6), unknown (2)
1
 English laurel (Prunus laurocerasus) no growth
2
 Rhododendron P. gonapodyides (6), Pythium spp. (2)
2
 Viburnum davidii unknown (2)
3
 Rhododendron P. gonapodyides (8), unknown (4)
3
 English ivy (Hedera helix ) P. gonapodyides (2), unknown (1)
4
 Rhododendron P. gonapodyides (7), unknown (2)
4
 Rhododendron (unknown hybrid) P. gonapodyides (1), unknown (1)
5
 Rhododendron
5
 English laurel (Prunus laurocerasus)
6
 Rhododendron P. gonapodyides (6), unknown (2)
6
 English ivy (Hedera helix ) unknown (2)

 

Liberty Bay/Miller Bay water quality report Kitsap County Health District 2009

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Evans Creek


Evans Creek

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Site #1003

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First baiting at Evans Creek, Redmond, WA by Master Gardener volunteers on March 2, 2010

1003

Baiting period Bait leaf spp. Phytophthora spp. and others identified
1
 Rhododendron P. gonapodyides (7), unknown (1)
1
 Himalayan blackberry (Rubus armeniacus) P. gonapodyides (2)
2
 Rhododendron P. gonapodyides (4), Pythium litorale or Py. sterilum (1), unknown (3)
2
 Japanese knotweed (Polygonum cuspidatum) P. gonapodyides (2)
3
 Rhododendron P. gonapodyides (2), P. drechsleri (1), Pythium sp.(1), Py. litorale or Py. sterilum (2), Py. undulatum (1), unknown (2)
3
 English laurel (Prunus laurocerasus) fungus* (Ophiocordyceps crassispora, Cylindrocarpon obtusisporum, Nectria) (1), unknown (1)
4
 Rhododendron P. gonapodyides (4), P. cambivora (1), unknown (4)
4
 Pacific madrone (Arbutus menziesii) unknown (2)
5
 Rhododendron P. gonapodyides (7), unknown (4)
5
 Evergreen huckleberry (Vaccinium ovatum) P. gonapodyides (1), unknown (2)
6
 Rhododendron P. gonapodyides (8), unknown (6)
6
 Kousa dogwood (Cornus kousa) no organisms isolated

*probably endophytic in host leaf

Evans Creek Natural Area

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

First Creek


First Creek Site # 1004

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Baiting at First Creek March 31, 2010

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Environmental Microbiology class (TESC 378) from UW-Tacoma.

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Results

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Links

First Creek Watershed blog

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

How to help


How to help

Let us know how you can help monitor Washington streams for invasive Phytophthora pathogens. Your email will be added to a mailing list and you will receive notifications of volunteer and research opportunities for this project.

I am a

Student Teacher Master Gardener or other volunteer Natural resource professional Interested party

I am interested in

Placing and retrieving bait bags
Making bait bags
Lab work at WSU Puyallup – culturing leaf baits, identification, media preparation
Computer work – Mapping, database construction, web applications , data analysis
Collect or share data from existing monitoring or research project
Working on a research project
Volunteer coordination

My contact information:

Name
Phone
Mailing Address
email

Include other comments here such as activities not mentioned above, your interests, etc.

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Presentations-Development of a Community-based Monitoring Program for Invasive Phytophthora Species in Western Washington Streams


Development of a Community-based Monitoring Program for Invasive Phytophthora Species in Western Washington Streams

Thursday, August 27 2009

8:30 – 9:00 am. Registration.

9:00 – 9:15 am. Introductions and discussion of objectives/goals. Gary Chastagner and Marianne Elliott,WSU Puyallup

9:15 – 10:15 am. Overview of SOD in California and stream monitoring in Northern CA. Dave Rizzo, UC Davis (pdf, 16.5 mb)

10:15-10:30 am.Break

10:30-11:00 am. The national SOD stream monitoring program. Steve Oak, USDA Forest Service, Asheville, NC (pdf, 4 mb)

11:00-11:20 am. Monitoring for P. ramorum in WA nurseries. Cindy Cooper, WSDA (pdf, 7 mb)

11:20 – Noon. Stream monitoring for P. ramorum in western Washington. Karen Ripley, WA DNR (pdf, 6 mb)

Noon – 1:00 pm. Lunch (provided)

1:00-2:00 pm. Panel Discussion – Gary Chastagner moderator

2:00-2:30 pm. Stakeholder discussion (forestry, nursery association, compost/greenwaste, others)

2:30-3:00 pm. Establishment of a working group to write proposal – WSU

Adjourn

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013