Clarks Creek – headwaters
gary.maguireSite # 1007Baiting at Clarks Creek upstream, near fish hatchery by Biology 213 class from Pierce College. 4/15/10.
see results of the Shoe Experiment here |
Site # 1007Baiting at Clarks Creek upstream, near fish hatchery by Biology 213 class from Pierce College. 4/15/10.
see results of the Shoe Experiment here |
PHS students learning molecular identification techniques in the lab | ||
Site #1001First bait deployment March 3, 2010 by Puyallup High School students in Dave Wetzel’s class |
Baiting period | Bait leaf spp. | Phytophthora spp. and others identified |
1
|
Rhododendron | P. gonapodyides (6), Trichoderma spp. (1) |
1
|
Camellia | P. gonapodyides (1), unknown (1) |
2
|
Rhododendron | P. gonapodyides (4), unknown (4) |
2
|
Gold dust plant (Aucuba japonica ‘Variegata’) | Pythium litorale or Py. sterilum (2) |
3
|
Rhododendron | P. gonapodyides (6), unknown (5) |
3
|
Strawberry tree (Arbutus unedo) | Pythium sp. or Py. undulatum (1), unknown (1) |
4
|
Rhododendron | P. gonapodyides (5), P. gonapodyides “A” (4), P. cryptogea (1), unknown (1) |
4
|
Dwarf cherry laurel | P. gonapodyides (2), unknown (2) |
5
|
Rhododendron | P. gonapodyides (7), unknown (5) |
5
|
unknown (2) | |
6
|
Rhododendron | P. gonapodyides (4), unknown (8) |
6
|
Red tip Photinia | P. gonapodyides (2) |
Viewing Chum Salmon in Clark’s Creek
WDFW 2000 Clear/Clark’s Creek Basin Plan Pierce County 2005
Pink salmon in Clarks Creek near Puyallup NWIFC Blog Sept. 2007
This project provided baseline information about oomycetes in western Washington streams. Some very common species that were ubiquitous in all streams in large numbers were identified, as well as some more rare species only found in certain streams. Several putative new species were also detected. The table below shows the locations of western Washington streams monitored in this study. Simpson’s diversity index (D) was calculated for each site based on the number of isolates and species of oomycetes detected. Values close to 0 indicate higher species diversity.
Stream ID | Name | County | Land use | Total number of isolates | Total number of species | Simpson’s diversity index (D) |
1101 | Packard Creek* | Clark | Agriculture/residential |
25 |
11 |
0.1333 |
1102 | Clarks Creek | Pierce | Agriculture |
9 |
6 |
0.1111 |
1103 | Clarks Creek | Pierce | Fish hatchery, residential |
15 |
8 |
0.2095 |
1104 | Woodard Bay | Thurston | Conservation area, brackish |
29 |
5 |
0.6823 |
1105 | Goldsborough Creek | Mason | Forest/residential |
31 |
7 |
0.428 |
1106 | North Creek | Snohomish | Suburban/residential |
22 |
9 |
0.0996 |
1107 | Clear Creek | Kitsap | Urban/commercial |
20 |
8 |
0.1526 |
1108 | Peters Creek | King | Urban/commercial |
22 |
6 |
0.2035 |
1109 | Vance Creek | Mason | Undisturbed forest |
25 |
4 |
0.3833 |
1110 | Thea Foss Waterway | Pierce | Formerly industrial, heavily polluted, brackish |
39 |
3 |
0.5196 |
1112 | Wollochet Creek* | Pierce | Suburban/residential, ditch adjacent to former nursery site |
23 |
5 |
0.332 |
1113 | Rosedale Creek* | Pierce | Suburban/residential |
16 |
8 |
0.1417 |
* Known to contain Phytophthora ramorum from WSDA or DNR baiting
King County
Mason County
Pierce County
Thurston County
Clark County
The most commonly isolated group of Oomycetes was Pythium belonging to Clade B2. There was little difference among hosts, except for reed canarygrass from which only one Pythium sp. was isolated. Saprolegnia was isolated from three hosts. Two Phytophthoras belonging to Clade 6 were isolated, and these, as well as Pythiums in Clade B2, were commonly found at the other freshwater sites sampled in this study. Phylogenetic trees from sequence data obtained in this study:
Phytophthora | Pythium | Saprolegnia |
Host | species isolated | group or clade | Riparian/forest | # isolates | |
False lily of the valley | Maianthemum dilatatum | Fusarium spp. | fungus |
F
|
2
|
False lily of the valley | Maianthemum dilatatum | Phytophthora taxon ‘salixsoil’ | 6 |
F
|
1
|
False lily of the valley | Maianthemum dilatatum | Pythium diclinum/lutarium | B2 |
F
|
5
|
Grand fir | Abies grandis | Phytophthora taxon ‘pgchlamydo’ | 6 |
F
|
1
|
Grand fir | Abies grandis | Pythium aquatile | B2 |
F
|
1
|
Grand fir | Abies grandis | Pythium diclinum/lutarium | B2 |
F
|
7
|
Licorice fern | Polypodium glycorrhiza | Mucor hiernalis | zygomycete |
F
|
1
|
Licorice fern | Polypodium glycorrhiza | Pythium aquatile | B2 |
F
|
1
|
Licorice fern | Polypodium glycorrhiza | Pythium diclinum/lutarium | B2 |
F
|
2
|
Licorice fern | Polypodium glycorrhiza | Pythium oopapillum | B2 |
F
|
1
|
Rhododendron | Rhododendron ‘Nova Zembla’ | Pythium aquatile | B2 |
F
|
1
|
Rhododendron | Rhododendron ‘Nova Zembla’ | Pythium diclinum/lutarium | B2 |
F
|
5
|
Rhododendron | Rhododendron ‘Nova Zembla’ | Saprolegnia parasitica | Saprolegnia |
F
|
1
|
Red alder | Alnus rubra | Pythium diclinum/lutarium | B2 |
R
|
5
|
Red alder | Alnus rubra | Pythium oopapillum | B2 |
R
|
2
|
Red alder | Alnus rubra | Pythium undulatum | H |
R
|
1
|
Reed canary grass | Phalaris arundinacea | Pythium intermedium | F |
R
|
8
|
Salmonberry | Rubus spectabilis | Pythium diclinum/lutarium | B2 |
R
|
8
|
Salmonberry | Rubus spectabilis | Pythium oopapillum | B2 |
R
|
1
|
Salmonberry | Rubus spectabilis | Saprolegnia parasitica | Saprolegnia |
R
|
1
|
Skunk cabbage | Lysichiton americanum | Mortierella sp. | zygomycete |
R
|
1
|
Skunk cabbage | Lysichiton americanum | Pythium diclinum/lutarium | B2 |
R
|
3
|
Skunk cabbage | Lysichiton americanum | Saprolegnia diclina | Saprolegnia |
R
|
1
|
Allmendinger Center, WSU Puyallup
Presentations
stream monitoring mtg july 29 2010 KPC2 – Marianne & Katie
Looking for Phytophthora ramorum – Lucy Rollins
Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013
The Clarks Creek Watershed is located in Puyallup. It contains a mixture of land uses and supports runs of steelhead trout, Chinook, pink, Coho, and chum salmon. Pollution from microbial contamination (bacteria and other pathogens) has been detected in high levels within Clarks Creek. The creek has been classified as a 303 (d) for fecal coliform. Water molds such as Phytophthora and Pythium species have also been detected in the creek. Invasive Phytophthora species such as P. ramorum can cause serious damage to native forests and to the nursery and forest products industries. P. ramorum has not been found in Clarks Creek but is present in other streams in western Washington. Early detection of these organisms will allow for rapid response and reduce their impacts to the ecosystems. Another water mold, Saprolegnia, is common on fish raised in hatcheries and could affect populations of native amphibians if present in high enough numbers.
WSU monitoring on Clarks Creek
2010
Downstream 1001 – Puyallup HS
Near headwaters 1007 – Pierce College Biology 213
2011
Downstream 1102 – Puyallup HS
Near headwaters