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Sudden Oak Death Horticulture

2011 Stream Monitoring Results


2011 Stream Monitoring Results

This project provided baseline information about oomycetes in western Washington streams. Some very common species that were ubiquitous in all streams in large numbers were identified, as well as some more rare species only found in certain streams. Several putative new species were also detected. The table below shows the locations of western Washington streams monitored in this study. Simpson’s diversity index (D) was calculated for each site based on the number of isolates and species of oomycetes detected. Values close to 0 indicate higher species diversity.

1108_DSC07178_350

Stream ID Name County Land use Total number of isolates Total number of species Simpson’s diversity index (D)
1101 Packard Creek* Clark Agriculture/residential

25

11

0.1333

1102 Clarks Creek Pierce Agriculture

9

6

0.1111

1103 Clarks Creek Pierce Fish hatchery, residential

15

8

0.2095

1104 Woodard Bay Thurston Conservation area, brackish

29

5

0.6823

1105 Goldsborough Creek Mason Forest/residential

31

7

0.428

1106 North Creek Snohomish Suburban/residential

22

9

0.0996

1107 Clear Creek Kitsap Urban/commercial

20

8

0.1526

1108 Peters Creek King Urban/commercial

22

6

0.2035

1109 Vance Creek Mason Undisturbed forest

25

4

0.3833

1110 Thea Foss Waterway Pierce Formerly industrial, heavily polluted, brackish

39

3

0.5196

1112 Wollochet Creek* Pierce Suburban/residential, ditch adjacent to former nursery site

23

5

0.332

1113 Rosedale Creek* Pierce Suburban/residential

16

8

0.1417

* Known to contain Phytophthora ramorum from WSDA or DNR baiting

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013

Stream monitoring sites 2011


Stream monitoring sites 2011

Snohomish County

1106 – North Creek

King County

Kitsap County

Mason County

2011 monitoring sites

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013

Results of BOB sampling at Clarks Creek


Results of BOB sampling at Clarks Creek

The most commonly isolated group of Oomycetes was Pythium belonging to Clade B2. There was little difference among hosts, except for reed canarygrass from which only one Pythium sp. was isolated. Saprolegnia was isolated from three hosts. Two Phytophthoras belonging to Clade 6 were isolated, and these, as well as Pythiums in Clade B2, were commonly found at the other freshwater sites sampled in this study. Phylogenetic trees from sequence data obtained in this study:

Phytophthora Pythium Saprolegnia

 

Host species isolated group or clade Riparian/forest # isolates
False lily of the valley Maianthemum dilatatum Fusarium spp. fungus
F
2
False lily of the valley Maianthemum dilatatum Phytophthora taxon ‘salixsoil’ 6
F
1
False lily of the valley Maianthemum dilatatum Pythium diclinum/lutarium B2
F
5
Grand fir Abies grandis Phytophthora taxon ‘pgchlamydo’ 6
F
1
Grand fir Abies grandis Pythium aquatile B2
F
1
Grand fir Abies grandis Pythium diclinum/lutarium B2
F
7
Licorice fern Polypodium glycorrhiza Mucor hiernalis zygomycete
F
1
Licorice fern Polypodium glycorrhiza Pythium aquatile B2
F
1
Licorice fern Polypodium glycorrhiza Pythium diclinum/lutarium B2
F
2
Licorice fern Polypodium glycorrhiza Pythium oopapillum B2
F
1
Rhododendron Rhododendron ‘Nova Zembla’ Pythium aquatile B2
F
1
Rhododendron Rhododendron ‘Nova Zembla’ Pythium diclinum/lutarium B2
F
5
Rhododendron Rhododendron ‘Nova Zembla’ Saprolegnia parasitica Saprolegnia
F
1
Red alder Alnus rubra Pythium diclinum/lutarium B2
R
5
Red alder Alnus rubra Pythium oopapillum B2
R
2
Red alder Alnus rubra Pythium undulatum H
R
1
Reed canary grass Phalaris arundinacea Pythium intermedium F
R
8
Salmonberry Rubus spectabilis Pythium diclinum/lutarium B2
R
8
Salmonberry Rubus spectabilis Pythium oopapillum B2
R
1
Salmonberry Rubus spectabilis Saprolegnia parasitica Saprolegnia
R
1
Skunk cabbage Lysichiton americanum Mortierella sp. zygomycete
R
1
Skunk cabbage Lysichiton americanum Pythium diclinum/lutarium B2
R
3
Skunk cabbage Lysichiton americanum Saprolegnia diclina Saprolegnia
R
1

Pierce College BOB 2011_allhosts

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

2010 Pilot Study Results


2010 Pilot Study Results

Long term sites – monitored for 6 two week periods between March 2 2010 and May 19 2010
Intensively sampled sites – monitored for one two week period with more samples collected
Student lab projects

DSC05649300 puget crk

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013

2010 pilot study results and planning for 2011 monitoring


2010 pilot study results and planning for 2011 monitoring

July 29, 2010 10 AM – 2 PM

Allmendinger Center, WSU Puyallup

How to get here

Agenda

Minutes

Presentations

stream monitoring mtg july 29 2010 KPC2 – Marianne & Katie

Looking for Phytophthora ramorum – Lucy Rollins

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013

Clarks Creek – Stream Monitoring Pilot Study


Clarks Creek

The Clarks Creek Watershed is located in Puyallup. It contains a mixture of land uses and supports runs of steelhead trout, Chinook, pink, Coho, and chum salmon. Pollution from microbial contamination (bacteria and other pathogens)  has been detected in high levels within Clarks Creek. The creek has been classified as a 303 (d) for fecal coliform.  Water molds such as Phytophthora and Pythium species have also been detected in the creek. Invasive Phytophthora species such as P. ramorum can cause serious damage to native forests and to the nursery and forest products industries.  P. ramorum has not been found in Clarks Creek but is present in other streams in western Washington. Early detection of these organisms will allow for rapid response and reduce their impacts to the ecosystems. Another water mold, Saprolegnia, is common on fish raised in hatcheries and could affect populations of native amphibians if present in high enough numbers.

WSU monitoring on Clarks Creek

2010

Downstream 1001 – Puyallup HS

Near headwaters 1007 – Pierce College Biology 213

2011

Downstream 1102 – Puyallup HS

Near headwaters


More about Clarks Creek: Clarks Creek Initiative

 

DSC05814_350
Clarks Creek near headwaters

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Results 2010


Results

2010 Pilot Study Results But what does it mean? Learn more about Phytophthora and Pythium species here.

DSC07281_350

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013

Sites 2010


2010 Stream monitoring pilot study

Clarks Creek 1007 Evans Creek 1003 Anderson Creek 1002 Clarks Creek 1001 Dogfish Creek 1006

Pierce County

Whatcom County

King County

Kitsap County

map of sites jpg sm

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Information for Volunteers 2010


Information for Volunteers

Methodology
Field methods, bait deployment and retrieval
Datasheets
Administrative
For minors under the age of 18:
 

DSC05275 sm

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA Last updated January 2, 2013