WSU Puyallup
Ornamental Plant Pathology
Inoculation of Noble fir seedlings with Phytophthora and Pythium spp.
For her senior project, a student at Bellarmine High School, Tacoma, is testing several isolates of Phytophthora and Pythium spp. to determine whether they cause disease on Noble fir seedlings. Noble fir (Abies procera) is commonly grown as a Christmas tree in western Washington and some growers irrigate using local stream water. Some of the test organisms have been collected from streams in western WA in the 2011 stream monitoring program. Others are known pathogens of Noble fir and are included as standards or to obtain more information about their behavior on roots.
Test organisms will be selected from this list:
| Sample # | Isolate # | Species | Origin |
|
1 |
1106-BP1-L2A | Pythium undulatum* | Stream bait, North Crk, Snohomish Co. |
|
2 |
1106-BP1-L4B | Phytophthora taxon “PGchlamydo”* | Stream bait, North Crk, Snohomish Co. |
|
3 |
109-0045 | Phytophthora cryptogea* | Noble fir roots, Puyallup, WA |
|
4 |
109-0038 | Phytophthora gonapodyides* | Noble fir roots, Puyallup, WA |
|
5 |
1106-BP1-L3B | Phytophthora taxon ‘salixsoil’* | Stream bait, North Crk, Snohomish Co. |
|
6 |
109-0049 | Phytophthora cambivora | Noble fir roots, Puyallup, WA |
|
7 |
P720 | Phytophthora cinnamomi* | Pacific madrone roots, CA |
|
8 |
1105-BP1-L3A | Pythium sp. #1* | Stream bait, Goldsborough Crk, Mason Co. |
|
9 |
109-0030 | Phytophthora citricola | Noble fir roots, Puyallup, WA |
|
10 |
109-0055 | Phytophthora megasperma* | Noble fir roots, Puyallup, WA |
|
11 |
1112-BP2-L2B | Pythium anandrum* | Stream bait, Wollochet Crk, Pierce Co. |
|
12 |
1112-BP1-L2B | Phytophthora inundata* | Stream bait, Wollochet Crk, Pierce Co. |
|
13 |
1102-BP1-L5B | Pythium grandisporangium | Stream bait, Clarks Crk, Pierce Co. |
|
14
|
1103-BP3-L4A | Phytophthora taxon “oaksoil”* | Stream bait, Clarks Crk, Pierce Co. |
*Used in Trial 1