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Washington State University
WSU Puyallup Ornamental Plant Pathology

The Sentinel Plant Network


The Sentinel Plant Network

sm191-9188_IMGThe American Public Gardens Association (APGA) is working with the National Plant Diagnostic Network to develop a Sentinel Plant Network, under a grant funded by USDA-APHIS under the 2008 Farm Bill.

APGA recently launched a SPN web page that provides an overview of the program, and its mission, the benefits of participation and information on how member gardens can get involved.

APGA has been working closely with NPDN to develop two “train-the-trainer” modules for public gardens. The first module addresses the impact of plant pests and pathogens and the importance of early detectors as well as providing an overview of the SPN, its primary partners, and the role of participating gardens. The second module, which is focused on the “best practices” of pest /pathogen scouting and reporting and outlines the proper methods of sample collection and submission, will provide valuable instruction to public garden professionals and volunteers. Gardens participating in SPN will be encouraged to use this module in classes offered to the general public, thereby giving them the necessary training to be effective citizen scientists contributing to the early detection of potential threats. Preliminary versions of these modules are expected prior to APGA’s conference in June and will undergo beta testing over the summer before being integrated into the SPN regional professional development workshops this fall and winter.

APGA is also developing a freestanding website marketed towards kids and educators and will work with a curriculum specialist to develop a teacher / parent guide to these resources.

 

 

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Inoculation of Noble fir seedlings with Phytophthora and Pythium spp.


Inoculation of Noble fir seedlings with Phytophthora and Pythium spp.

NF_IMG_0470_350For her senior project, a student at Bellarmine High School, Tacoma, is testing several isolates of Phytophthora and Pythium spp. to determine whether they cause disease on Noble fir seedlings. Noble fir (Abies procera) is commonly grown as a Christmas tree in western Washington and some growers irrigate using local stream water. Some of the test organisms have been collected from streams in western WA in the 2011 stream monitoring program. Others are known pathogens of Noble fir and are included as standards or to obtain more information about their behavior on roots.

 

 

 

Test organisms will be selected from this list:

Sample # Isolate # Species Origin

1

1106-BP1-L2A Pythium undulatum* Stream bait, North Crk, Snohomish Co.

2

1106-BP1-L4B Phytophthora taxon “PGchlamydo”* Stream bait, North Crk, Snohomish Co.

3

109-0045 Phytophthora cryptogea* Noble fir roots, Puyallup, WA

4

109-0038 Phytophthora gonapodyides* Noble fir roots, Puyallup, WA

5

1106-BP1-L3B Phytophthora taxon ‘salixsoil’* Stream bait, North Crk, Snohomish Co.

6

109-0049 Phytophthora cambivora Noble fir roots, Puyallup, WA

7

P720 Phytophthora cinnamomi* Pacific madrone roots, CA

8

1105-BP1-L3A Pythium sp. #1* Stream bait, Goldsborough Crk, Mason Co.

9

109-0030 Phytophthora citricola Noble fir roots, Puyallup, WA

10

109-0055 Phytophthora megasperma* Noble fir roots, Puyallup, WA

11

1112-BP2-L2B Pythium anandrum* Stream bait, Wollochet Crk, Pierce Co.

12

1112-BP1-L2B Phytophthora inundata* Stream bait, Wollochet Crk, Pierce Co.

13

1102-BP1-L5B Pythium grandisporangium Stream bait, Clarks Crk, Pierce Co.
14
1103-BP3-L4A Phytophthora taxon “oaksoil”* Stream bait, Clarks Crk, Pierce Co.

*Used in Trial 1

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Data analysis and report writing basics


Data analysis and report writing basics

DSC07459sm300Now you have data, what to do with it?

This manual provides good information about designing experiments, data exploration, and statistical tests. You will need Microsoft Excel and the data analysis tool pack installed.

How to write a scientific article – each journal will have its own format, but the basic ingredients can be found at this link:

How to write a paper in scientific journal style and format

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

Student Stream Monitoring Projects


Student Projects

Students from local high schools, community colleges, and universities can participate in research projects related to Phytophthora and other oomycetes.

Ideas for student projects

Other student projects


Some projects done by students in 2012:

Pierce College, Puyallup, independent studies

Temperature growth rates of Phytophthora and Pythium species isolated from western Washington streams.

Temperature effects on root infection of Noble and Frasier fir by Phytophthora species isolated from western Washington streams and soils.

Effects of gypsum on sporulation of Phytophthora species.

Biocontrol of Phytophthora using various bark mulch treatments.

Bellarmine HS, Tacoma WA, senior projects

Fungus death match! – Antagonism of Trichoderma spp. to selected Phytophthora spp.


Some projects done by students in 2011:

Green River Community College independent studies

Screening a collection of Trichoderma spp. for antagonism to Phytophthora ramorum.

Pierce College, Puyallup, Biology 213

Stream sampling using “bait in a bottle” method at Clarks Creek

The “shoe experiment” year 2

Bellarmine HS, Tacoma WA, senior projects

Testing isolates of Phytophthora and Pythium for pathogenicity to Noble fir roots

Survival of P. ramorum in water from various streams in western WA


Some projects done by students in 2010:1103_DSC07359_350

 

Effects of salinity on P. ramorum growth, survival, and sporulation (UW Tacoma, Environmental Microbiology)

“The shoe experiment” – testing various disinfectants for sanitizing the bottoms of shoes (Pierce College, Puyallup, Biology 213)


 

Learn more about Phytophthora here

 

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013

2012-Students


2012 Student projects

DSC09635_350Pierce College, Puyallup, independent studies

Temperature growth rates of Phytophthora and Pythium species isolated from western Washington streams.

Temperature effects on root infection of Noble and Fraser fir by Phytophthora species isolated from western Washington streams and soils.

Effects of gypsum on sporulation of Phytophthora species.

Biocontrol of Phytophthora using various bark mulch treatments.

Bellarmine HS, Tacoma WA, senior projects

Fungus death match! – Antagonism of Trichoderma spp. to selected Phytophthora spp.

Does exposure to cold increase severity of leaf blight caused by the fungus Phacidiopycnis washingtonensis on Pacific madrone (Arbutus menziesii)?

Dominican University of California, summer internship

A study of root infection of Viburnum by Phytophthora ramorum.

Washington State University, summer internship

Isolation and screening of biocontrol organisms found in Phytophthora-suppressive bark mulch substrates.

 

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013