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Washington State University
WSU Puyallup Ornamental Plant Pathology

PCR for DNA sequence analysis and identification of Oomycetes

Setting up PCR 5 24 2011 (click on photos to see larger photo).


Katie Coats, molecular biologist, showing students pipetting skills. Accurate pipetting is very important in DNA analysis.


Samples which were isolated onto V8 agar in pure culture will be tested.


A master mix of chemicals used in PCR is prepared.


Samples are centrifuged to mix thoroughly.



A sterile pipet tip is scraped across the surface of a culture to obtain a sample for PCR.


Then the chemicals are pipetted into microtubes for each sample.


All samples are placed in the thermocycler for PCR, which runs about 2 hrs. Then the PCR product is sent off for sequencing.

Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013