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PPO Home > WSU Sudden Oak Death Program > Monitoring > Volunteer Stream Monitoring Program > 2011 study > Stream monitoring sites 2011 > Clarks Creek Headwaters 1103 > Stream sampling using “bait in a bottle” method at Clarks Creek > PCR for DNA sequence analysis and identification of Oomycetes

PCR for DNA sequence analysis and identification of Oomycetes

Setting up PCR 5 24 2011 (click on photos to see larger photo).

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Katie Coats, molecular biologist, showing students pipetting skills. Accurate pipetting is very important in DNA analysis.
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Samples which were isolated onto V8 agar in pure culture will be tested.
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A master mix of chemicals used in PCR is prepared.
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Samples are centrifuged to mix thoroughly.

 

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A sterile pipet tip is scraped across the surface of a culture to obtain a sample for PCR.
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Then the chemicals are pipetted into microtubes for each sample.
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All samples are placed in the thermocycler for PCR, which runs about 2 hrs. Then the PCR product is sent off for sequencing.
Contact: Gary Chastagner, 253-445-4528 | WSU Puyallup Research & Extension Center, 2606 West Pioneer, Puyallup, WA, 98371-4998 USA
Last updated January 2, 2013