Results of BOB sampling at Clarks Creek

The most commonly isolated group of Oomycetes was Pythium belonging to Clade B2. There was little difference among hosts, except for reed canarygrass from which only one Pythium sp. was isolated. Saprolegnia was isolated from three hosts. Two Phytophthoras belonging to Clade 6 were isolated, and these, as well as Pythiums in Clade B2, were commonly found at the other freshwater sites sampled in this study. Phylogenetic trees from sequence data obtained in this study:

PhytophthoraPythiumSaprolegnia
Host common name Host Latin namespecies isolatedgroup or cladeRiparian/forest# isolates
False lily of the valleyMaianthemum dilatatumFusarium spp.fungus
F
2
False lily of the valleyMaianthemum dilatatumPhytophthora taxon ‘salixsoil’6
F
1
False lily of the valleyMaianthemum dilatatumPythium diclinum/lutariumB2
F
5
Grand firAbies grandisPhytophthora taxon ‘pgchlamydo’6
F
1
Grand firAbies grandisPythium aquatileB2
F
1
Grand firAbies grandisPythium diclinum/lutariumB2
F
7
Licorice fernPolypodium glycorrhizaMucor hiernaliszygomycete
F
1
Licorice fernPolypodium glycorrhizaPythium aquatileB2
F
1
Licorice fernPolypodium glycorrhizaPythium diclinum/lutariumB2
F
2
Licorice fernPolypodium glycorrhizaPythium oopapillumB2
F
1
RhododendronRhododendron ‘Nova Zembla’Pythium aquatileB2
F
1
RhododendronRhododendron ‘Nova Zembla’Pythium diclinum/lutariumB2
F
5
RhododendronRhododendron ‘Nova Zembla’Saprolegnia parasiticaSaprolegnia
F
1
Red alderAlnus rubraPythium diclinum/lutariumB2
R
5
Red alderAlnus rubraPythium oopapillumB2
R
2
Red alderAlnus rubraPythium undulatumH
R
1
Reed canary grassPhalaris arundinaceaPythium intermediumF
R
8
SalmonberryRubus spectabilisPythium diclinum/lutariumB2
R
8
SalmonberryRubus spectabilisPythium oopapillumB2
R
1
SalmonberryRubus spectabilisSaprolegnia parasiticaSaprolegnia
R
1
Skunk cabbageLysichiton americanumMortierella sp.zygomycete
R
1
Skunk cabbageLysichiton americanumPythium diclinum/lutariumB2
R
3
Skunk cabbageLysichiton americanumSaprolegnia diclinaSaprolegnia
R
1
More than half of the species identified from all above hosts were from Pythium diclinum/lutarium B2. Nearly half of what was left was Pythium intermedium F. In order, the next three get smaller: Pythium oopapillum B2, Pythium aquatile B2, and Saprolegnia parasitica. The last four are smallest and show about the same amount: Pythium undulatum H, Saprolegnia diclina, Phytophthora taxon 'pgchlamydo" 6, and Phytophthora taxon "salixsoil" 6

WSU Puyallup Research & Extension Center
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Last updated 9/24/2025