WSU Puyallup
Ornamental Plant Pathology
Long term sites
Each site had two bait bags containing 10 leaves/sampling period. Bait leaves used were Rhododendron ‘Nova Zembla’ and a ‘wild card’ species of choice. Leaves were sliced in several locations along the midrib to allow for better colonization by Phytophthora. See individual sites for more detailed results. Of these ten leaves, five leaves were cultured and two isolates per leaf were identified using PCR-RFLP and sequencing if necessary, for a total of 10 samples per baiting period. Phytophthora gonapodiydes was found the most often. Unknowns are being sequenced.
1001 – Clarks Creek Downstream
1002 – Anderson Creek
1003 – Evans Creek
1006 – Dogfish Creek